Neurons form vast interconnected networks that are essential for brain function. They are connected via synapses that allow signals to pass from one neuron to the next. Synaptic plasticity is the modulation of connections between neurons in response to synaptic transmission, and is fundamental to learning and memory.
Research in our laboratory focusd on signalling pathways that are related to impaired synaptic plasticity and cognition. We have shown that mTOR, a critical regulator of protein synthesis, is overactivated in Fragile X mice and may account for aberrant synaptic plasticity. We further showed that PIKE (PI3K enhancer), an identified target of FMRP and key player that links mGluRs to mTOR, is elevated in Fragile X mice.
The goal of this study was to establish whether PIKE is a potential therapeutic target for the treatment of Fragile X. We hoped that by restoring PIKE expression to normal levels in Fragile X mice, we will be able to correct aberrant signalling, protein synthesis and synaptic plasticity.
We used two complementary genetic strategies to manipulate PIKE expression:
- acute knockdown of PIKE by injection of lentiviral shRNA directly into the brain of live animals and
- creating transgenic mice in which the PIKE gene is knocked out.
Kirsty Sawicka, PhD
Sho Fujisawa, PhD
FRAXA Postdoctoral Fellow
Yukihiro Takayasu, PhD
FRAXA Postdoctoral Fellow (2006)